Strokina A. Thiamine phosphate hydrolase activity of neural cells plasma membrane

Object: Enzymatic hydrolysis of thiamine triphosphate by neural cells plasma membranes and thiamine-binding protein. Aim: to investigate the structure and functionality of membrane-bound thiamine phosphate hydrolase as a part of thiamine-binding protein in neural cell plasma membranes. Methods: biochemical and molecular biology. It was shown that the active site of the neural thiamine triphosphate hydrolyzing enzyme is associated with the inside plasma membrane. It was found two different protein sites responsible for the thiamine binding and for thiamine phosphates hydrolysis by thiamine-binding protein of synaptic membranes. It was discovered that membrane-bound thiamine triphosphatase is the structural part of thiamine-binding protein, namely its subunit with molecular mass 35 kDa, and homologous to the soluble enzyme. Scope: organizations of medical and biological profile.