Asmolkova V. Biochemical mechanisms of N-acylethanolamines regulation of functional characteristics of cells in culture

Object: primary and transformed cell cultures. Aim: to study biochemical mechanisms of influence of saturated N-stearoilethanolamine (NSE), monounsaturated oleoilethanolamine (OEA) and multicomponent N-acylethanolamines mixture of more then twenty saturated and unsaturated N-acylethanolamones (NAE) on primary and transformed cells proliferation in culture. Methods: cell culture, NAE chemical synthesis, optical microscopy, flow cytometry, petch-clamp, cytochemical determination of the enzyme activity, exposure to ionizing radiation, thin layer chromatography, spectrophotometry, the statistical analysis. It is shown that NAE influence on quantity of primary and transformed cells is determined by unsaturation level of studied substances and also is directed differently for normal and transformed cell cultures. It is indicated that NSE, ОЕА and multicomponent NAEs mixture effects are implemented by the regulation of phospholipid composition of cell plasma membranes. The ability of NSE and ОЕА to change cells membrane potential via partial block of sodium channels in vitro is shown. It is determined that NAE modulate activity of mitochondrial enzymes, which can be the part of NAEs effects realization. Omni directional character of NAEs effects on normal and transformed cells functioning can be the base for new anticancer drugs development in medical and biological institutions.