Zhylischych Y. Biochemical mechanisms of cadmium impact on oxygen-transport function of blood

The object - biochemical mechanisms of influence of cadmium and vitamin E on oxygen-transport function of animals blood The purpose - to investigate the peculiarities of influence of Cd2+ in the conditions of long-term intake in the form of CdCl2 (3 mg/kg) on oxygen-transport function of blood of animals (rabbits, white rats); to analyze biochemical mechanisms that underlie adaptational response of erythron system to the long-term effects of Cd2+; to explore a possibility of preventing the harmful effects of cadmium in blood cells by vitamin E administration. Methods - biochemical (spectrophotometry), cytological, statistical. Novelty - for the first time biochemical mechanisms of the disturbances in oxygen-transport function of blood and functional activity of erythroid cells in rats and rabbits in the conditions of 28-day peroral intake in a form of CdCl2 (3 mg/kg) were analyzed. It was established that cadmium provoked suppression of hemoglobin synthesis, accelerated ageing of red blood cells and impairment of membranes stability, in consequence of the stimulation of lipid peroxidation, and the inhibiting activities of enzymes of antioxidant system and pentose phosphate pathway. The peculiarities in adaptational response of erythron system to long-term period of Cd2+ intake by animals were determined. For the first time, the differences in metabolic response of erythroid cells of bone marrow and blood on cadmium poisoning, and features in susceptibility of cells of rats and rabbits to oxidative stress and vitamin E were shown. The corrective influence of vitamin E on oxygen-transport function of blood in the conditions of long-term intake of cadmium was proved.