Blonsky R. Cell-based therapy of degenerative injuries of tendons. (experimental study)

The dissertation is focused on a topical question of studying the impact of autologous mesenchymal stem cells, autologous fibroblasts, and autologous platelet-rich plasma in restoration of a tendon affected by degenerative pathology. This paper contains analysis and comparative evaluation of the outcomes of pathomorphological, biomechanical, FTIR spectroscopic, biochemical, and microscopic luminescent study. Based on the analysis of outcomes of modeling of degenerative tendon injury in 60 sexually mature male rats by burying Diprosan solution it has been established that thrice-repeated burying of this solution into the tendon induces in the latter the offset of the degenerative dystrophic process. The changes determined in the process, metabolic, biomechanical and histological ones, could serve as the criteria for evaluation of course of the degenerative - dystrophic process, and the proposed by us procedure can be applied as a model of degenerative tendon injury in further scientific studies. As a result of the analysis of data of the experimental study on application of autologous mesenchymal stem cells, autologous fibroblasts, and autologous platelet-rich plasma to treat degenerative tendon injuries in 301 sexually mature male rats, it has been proven that intratendinous administration of the culture of autologous mesenchymal stem cells and of the culture of autologous fibroblasts in degenerative tendon's injury, in the most effective way contributes to the reparative restoration of the structural organization of the tissue at the 7th and 21st day giving practically full restoration of tendon tissue structure at the 45th day of observation; the application of the autologous platelet-rich plasma was characterized by weaker regenerative effect, in this case pathological process in the control group of rats tended to progress.