Chechui H. Activity of glyoxylate cycle key enzymes and gluconeogenesis of Glycine max L. under Сobalt and Сadmium ions

3. The object of study: regulation of key enzymes of glyoxylate cycle and enzymes, providing the conversion of fatty acids into carbohydrates under the influence of heavy metals ions. Aim of study: determine the effect of Co2+ and Cd2+ ions on the enzyme activity of glyoxylate cycle and gluconeogenesis in seeds of Glycine max L. during germination Methods: the basis spectrophotometry methods (activity of isocitrate lyase, malate synthase, malate dehydrogenase, succinate dehydrogenase, aspartate- and alaninaminotransferase), thitrometry methods (check indexes), photocolourmetryc (contents of protein), differentional centrifugation and method of variation statistics). Theoretical and practical results, novelty: Is dedicated to a study of key enzymes activity of glyoxylate cycle and gluconeogenesis under the influence of heavy metals, Co2+ and Cd2+ ions, in Glycine max L. of germination. It shown, that the key enzymes of glyoxylate cycle isocitratelyase leading to different dynamics of activity level changes in given enzyme for five days of germination Glycine max L. under the influence of Co2+ and Cd2+ ions and reflect participation of regulatory systems in formation of organism's adaptation to the action of heavy metals compounds. As a result of the research significant influence of Co2+ and Cd2+ ions on key gluconeogenesis enzyme systems functioning have been proved as well as existence of powerful compensation mechanisms assisting in metabolism stability increase of Glycine max L. to stress conditions. Field of application: The results are a basic foundation for concrete ways and mechanisms of the effect of heavy metals in biological structures and their metabolic processes. The practical value of the thesis is to look towards the correction of violations to the general physiological condition of the body. Experimental generalizations of work can be applied in the educational process in teaching the course "Plant Physiology", "Biochemistry" and the special course "Resistance of Plants" on biological departments of higher educational institutions.