Shamro N. The influence of vitamins C and E on the activity of NO-synthase system and lipoperoxidation processes in the mucosal membrane of the large intestine in rats under conditions of stress and colitis

The dissertation is devoted to the evaluation of the influence of vitamins C and E under their isolated and combined action, their influence on the status of NO-synthase system, activity of lipoperoxidation processes and antioxidant defense enzymes in iNOS, COX-2 blockage and L-arginine introduction under conditions of adrenaline-induced stress and ulcerative colitis in rats. It was shown that the isolated action of vitamins C and E mainly cause the decrease of іNOS, nitrite anion content and activity of lipoperoxidation processes. The combined action of vitamins C and E did not cause the enhancement of their effects on the activity of NO-synthases and lipoperoxidation processes in mucosal membrane of the large intestine. The action of vitamin C under conditions of blockage of іNOS, COX-2 and L-arginine administration in adrenaline-induced stress and colitis is dominating in the regulation of NO-synthases activity ad did not cause significant changes of lipoperoxidation processes, SOD and catalase activity. The action of vitamin E on the activity of NO-synthases and lipoperoxidation processes was modeled dependently from inhibition of the activity of iNOS, COX-2 and L-arginine introduction. Inhibition of iNOS activity under conditions of vitamins C and E introduction may be the consequence of their antioxidant action.