Biletska L. Biochemical mechanisms of action of Cadmium and Plumbum on leukopoiesis and immune activity of leukocytes of white rats.

Object-biochemical mechanisms of action of Сadmium and Plumbum on leucopoiesis and immune function of white blood cells, correction of metabolic disorders in the cells of the immune system of vitamin E. Purpose - to find out the biochemical mechanisms of action of Сadmium cations and Plumbum in the form of CdCl2 and Pb(CH3COO)2 in functional activity of bone marrow cells, blood leukocytes and spleen of white rats under prolonged flow and under the corrective effect of vitamin E. Methods: biochemical (spectrophotometric), cytological, statistical. Novelty: It is shown that under the influence of prolonged and acute poisoning with Cd neutrophilic granulocytes content in animal blood increases, but their functional activity decreases with the duration of receipt of toxicant. At the same time content of lymphocytes decreased but in these cells is adaptive activation energy processes. Single parenteral administration CdCl2 resulted in the increase of neutrophilic granulocytes and reduction of lymphocytes within 10 days after injection. Shown that cadmium causes the change in functional activity of neutrophilic granulocytes - an increase of phagocytic index and phagocytic number on the 7th day and reduction of these indicators on the 14th day. Introduction Plumbum causes increased phagocytic index and phagocytic number of neutrophilic granulocytes in the 14 th day of the experiment. Prolonged administration of Pb and Cd cations leads to activation of lipid peroxidation processes in all cells studied and changes in antioxidant enzyme activity, as well as intensification of anaerobic catabolism of monosaccharides. It is accompanied by increasing activity of enzymes that catalyze several stages of glycolysis and pentose phosphate shunt. Vitamin E (?-tocopherol) administration resulted in reduction of TBA-reactive products in lymphocytes and neutrophilic granulocytes of animals intoxicated with Cd. Similar effects were found in leukocytes of animals injected with ?-tocopherol against gradual intoxication with Pb cations.