Kandaurova N. Ca2+-induced changes of membrane potential of myometrium mitochondria

Object - mitochondria of rat myometrium. Aim - to study Ca2+-induced changes of the mitochondrial membrane potential and the influence of the modulators of transmembrane Ca2+-exchange on it. Methods: preparative biochemistry, biochemical membranology, spectrophotometry, flow cytometry, confocal microscopy, photon-correlation spectroscopy, light and electron microscopy, kinetic and statistic analisys. Using potential- and Ca2+-sensitive fluorescent probes and flow cytometry on isolated mitochondria and permeabilized myocytes of rat uterus, the effects of Ca2+ and Mg2+ on mitochondrial membrane potential, the influence of calmodulin antagonists on the mitochondrial membrane polarization and the ionized Ca content, and the regularities of Ca2+-changes in depolarized mitochondria under the action of modulators of transmembrane Ca2+-exchange in these subcellurar structures were investigated. Scope - organizations of medical and biological profile.