Kondratiuk A. Regulation of haemostatic balance of blood components of fibrinolytic system.

The dissertation is devoted to the study of participation of individual proteins of fibrinolytic system in activation and inhibition of fibrinolysis to determine their role in maintaining a haemostatic balance and their possible use as markers of lower fibrinolytic potential of blood. Kinetic characteristics of activation reaction of plasminogen by t-PA in the presence of human fibrin have been found to be similar to those obtained with the usage of recombinant t-PA and bovine fibrin. This finding proves that the bovine fibrin can be used as an efficient cofactor of this reaction. It has been shown for the first time that alfa-2-antiplasmin does not affect the process of activation and hydrolysis of fibrin clot by plasminogen activated by catalytic number of Sk in the presence of fibrin, which means that activation of plasminogen by Sk occurs on the surface of the fibrin clot. The methods of determining content of functionally active plasminogen, t-PA and plasminogen activator inhibitor type 1 (PAI-1) in plasma using bovine fibrin are proposed. The t-PA activity decrease and PAI-1 level increase in plasma of patients with certain pathologies show the potential of their use as prognostic markers of thrombus formation risk and their key role in maintaining haemostatic balance.