Volkova Y. Peculiarities of oxidative stress and antioxidant system changes in brain and liver of pubertal rats during immobilization.

The object of study – free radical processes, enzymatic and low-molecular antioxidants in brain and liver of intact and immobilized rats of different ages. The purpose of the study: assessment of intensity of free radical processes and antioxidant system in the brain and liver of rats at different stages of puberty, in a short-term and long-term immobilization stress. Methods: spectrophotometry in the visible and near ultraviolet spectral region (determination of enzyme activity of first line of antioxidant defense, the content of lipid peroxidation products, oxidatively modified proteins, low-molecular antioxidants), fluorymetry (determination of 11-oxycorticosteroids), differential centrifugation (fractionation of brain and liver homogenates), the statistical analysis. Theoretical and practical results, novelty: the first established features of oxidative stress in brain and liver of pubertal rats in their short- and long-term immobilization. It has been shown that short-term immobilization in the liver of animals 1,5 - and 2-month-old, and in the brain of 1,5-months-old rats formed oxidative stress. Under conditions of prolonged immobilization oxidative stress in brain and liver occurs only in 1,5-months-old rats. Arising changes reflect the different contribution of oxidative stress in the adaptation of tissues of viscera to the damaging effect of strong stressors during puberty. At the stage of early puberty involvement of oxidative stress in protection of the brain and liver from stress damage is limited. Field of application: the obtained results extend the existing understanding about the mechanisms of reduction of resistance to the stressors action on the organism in the pubertal age. The results introduced in the lectures of the Department of Biological Chemistry Kharkiv National Medical University, Department of Clinical Biochemistry and Forensic Medical Toxicology Kharkiv Medical Academy of Postgraduate Education, and Department of Biological Chemistry and Laboratory Diagnostics Zaporіzhzhya State Medical University.