Kovalyova M. Characteristic of some metabolic indices of cell culture Dunaliella viridis at process of long-term cultivation.

The object of study – copper-sensitive culture and copper-resistant culture of microalgae Dunaliella viridis Teod. var. viridis f. euchlora strain IBASU-A N29. Purpose – to research regularity of chronological and replicative senescence of copper-sensitive and copper-resistant cultures of microalgae Dunaliella viridis. Methods: microscopical, method of thin-layer chromatography for analysis of lipids fraction, spectrophotometry, method of atomic absorption spectrometry for detection of copper content, statistical analysis. Theoretical and practical results, novelty: it was detected that DNA, triacylglycerids and ?-carotene contents are criteria of age-dependent metabolic changes in culture. In accordance with chosen criteria, it was revealed that subculture-10 is «young» culture, subculture-40 is «old» culture. It was pointed out that the changes of metabolic indices in microalgae cells in long-term cultivation are dependent on transplantation intensity of cultures and media composition (copper content). Copper-resistant culture has greater rate of replicative «senescence» than copper-sensitive culture. Regularities of chronological «senescence» of copper-sensitive culture and copper-resistant culture are different. Field of application: use of culture of microalgae D. viridis as experimental model in gerontological researches and for study of cells adaptation mechanisms to heavy metals ions. The choice of cultivation regimes of microalgae cultures for preservation of epigenetic stability and high productivity of cultures.