Yermolenko I. Atomic Force Microscopy and Simulation of Adhesion of Biopolymers on Solids

Based on an interdisciplinary approach in the studying of complex systems, we have developed a concept consisting of the use of Atomic Force Microscopy (AFM) in studying the parameters of adsorption and aggregation of blood proteins, such as fibrinogen, on solid surfaces. This allows us to evaluate the adhesion forces between the substrate and the probe, as well as between the substrate and the cells attached to the cantilever. The method based on using chemical cross-linking for improving adhesion forces between the first and subsequent layers of fibrinogen, which improves the density of molecular packing in the fibrinogen coating adsorbed on the implant surface is proposed. With the use of Atomic Force Microscopy, intact hydrated fibrin fibers were shown with cross-striations having an axial period of 24±1.6 nm. Computer simulation of the experimental images shows that the AFM patterns are in good agreement with the molecular dimensions and shapes detected by other methods. To improve the biocompatibility of inorganic implants with the human body, methods were developed based on protein coatings resulting in the reduction of the possibility of immune system recognition of the presence of a foreign body.