Shemedjuk N. Biochemical characteristic of extracts action of some medical plants: the indices valuation of oxidant and antioxidant state in vivo and induction of apoptosis tumour cells in vitro.

Object: influence extracts of A. montana, P. erecta, S. japonica on oxidant-antioxidant system blood and mice's hepatic, biochemical, morphologycal indications of blood, kidney, spleen, and mice's hepatic; influence of extracts on the growth, viable, antiproliferative activity, cell lines MDA-MB-231, 4T1, BALB 3T3 in vitro, apoptosis induction.Methods: biochemical, cytomorphological, cytochemical, microbiological, histological, and method's statistic.Thanks to the results we can be sure due to the possitility of the influence on tumour cells of extracts of plant. Extracts A. montana, P. erecta, S. japonica tested in vivo and it was determined their influence of the doses and ways of injection to mice. The extract of P. erecta of it's getting into the fodder of optimal dose has the action as antioxidant mean.It was also found out the most effective concentration of the extracts, and the the action in vitro are taken place of growth inhibition, viability, proliferative activity and the induction of apoptosis of tumour cells of lines MDA-MB-231, 4Т1 because of them. For the population of 4T1, proapoptic changes in morphology of some cells are typical by the action of extract concentration S. japonicа, P. erecta 2*10-5, 2*10-4 g/ml. For the population of 4T1, the rise of the quantity of giant cells is typical by the action of extract concentration P. erecta 2*10-5 g/ml. It was found out the fragmentation of DNA, and that's why the destruction of lines cells 4Т1 by apoptosis mechanism, which were cultivated in the presence of P. erecta, S. japonicа.