Zatsarinna Y. Ecological approaches to phytoremediation of Trifluralin contaminated soil

The thesis is devoted to investigation of accumulation and distribution of Trifluralin in the soil and plants (Valeriana officinalis L., Lophanthus anisatus L., Plantago major L., Tagetes signota Barti, Calendula officinalis L., Astragalus dasyanthus Pall., Echinacea purpurea L., Sіlybum marіanum L., Inula helenium L., Scutellaria baicalensis Georgi). As a result of the work, the migration and especially the distribution of Trifluralin in the soil (light loamy black soil with low humus content) were analyzed. The presence of Trifluralin residues in the overground and underground parts of medicinal plants was found; significant concentrations of Trifluralin residues were defined in raw materials for medicinal industry. The highest allowed concentration of Trifluralin was exceeded on soils where this herbicide was used during last years. The necessity of developing phytoremediation's methods for soils contaminated by Trifluralin for growing of medicinal plants was established. The vegetation trials for searching plants - perspective remediators of Trifluralin - were conducted. For trials the following species of plants were used: zucchini (Cucurbita pepo L.), bean (Phaseolus vulgaris L.), sunflower (Helianthus annuus L.), alfalfa (Medicago sativa L.) and soybean (Glycine max L.). For those plants the ability to herbicide's accumulation, biometrics and the intensity of lipid peroxidation as marker of physiological responses of plants to stress load were defined. It was found that all studied species showed rather high capacity of the Trifluralin accumulation from soil; that caused the remediation of soil. It is proved that the intensity of lipid peroxidation depends on the concentration of Trifluralin in the soil: increasing of the Trifluralin concentration in the soil causes the increasing of the lipid peroxidation intensity in the plants' leaf. It should be noted that there were the highest intensity of lipid peroxidation - in the leaf of zucchini, the meddle intensity of that indicator - in leaf of bean, and the lowest intensity of lipid peroxidation - in the leaf of sunflower. It was shown that the plants from Fabaceae (in particular bean) were the most promising for the phytoremediation of Trifluralin contaminated soils.