Panchyshyn O. Relationship between NO-synthase, endogenic prostaglandins and alpha-amylase of pancreas under conditions of experimental diabetes mellitus

Object of the research - activity of proinflammatory enzymes, pancreatic amylase and lipoperoxidation processes under conditions of experimental diabetes mellitus. The aim of the work - to determine changes of the L-Arginine/NO system, arginase, oxidative processes during correction of the proinflammatory enzymes (iNOS, COX-2 and 5-LOX) activity in the pancreatic tissue in relation to the activity of pancreatic alpha-amylase in blood plasma of the rats with streptozotocin-induced diabetes. Methods: biochemical (spectrophotometric, colorimetric), statistical. Novelty: conducted researches have shown a negative part of the proinflammatory enzymes (iNOS, COX-2 and 5-LOX) and positive effect of L-Arginine in pathochemical mechanisms and in endocrine-exocrine interconnections in pancreas under conditions of experimental diabetes mellitus. For the first time it has been indicated that during fortnight injection of L-Arginine, activity of iNOS and content of TBA-active products reduces, activity of arginase in the pancreatic tissue increases as well as concentration of L-arginine and activity of pancreatic amylase in the plasma of blood. It has been determined that two-week inhibition of COX-2 activity by celexocib under conditions of streptozotocin-induced diabetes leads to reduction of activity of iNOS, content of TBA-active products with a simultaneous increasing of arginase activity in the pancreatic tissue and activity of pancreatic amylase in the plasma of blood. For the first time it has been indicated that two-week combined inhibition of COX-2/5-LOX by 2А5DHT substance resulted in more significant reduction of the activity of iNOS, content of TBA-active products in the pancreatic tissue and more distinct increasing of the pancreatic amylase activity in the plasma of blood in comparison with indicators of COX-2 inhibition under conditions of streptozotocin-induced diabetes. Obtained results can be used in practical endocrinology - at administration of nonsteroidal antiinflammatory drugs for patients with diabetes mellitus. L-Arginine can be used for correction of diabetes mellitus type 1 patient's health condition. Results of combined inhibition of COX-2/5-LOX prove administration of inhibitors of the given group of enzymes for patients suffering from diabetes mellitus.