Ustjansky O. Regulation of cathepsins activity by thiamine and its metabolites.

The object of the research is mechanisms of regulation of cathepsins activity in morphologically different organs and at the level of the purified enzyme. The purpose of the research is to study the influence of thiamine its metabolites on activity of cathepsin-B-like and cathepsin-L-like enzymes in vivo and on the partly purified cathepsin L. The methods of the research. There were applied methods of preparative biochemistry, spectrophotometry in visible and near ultroviolet regions of the spectrum (determination of activity of proteolytic enzymes), and also statistical aanalysis.Teoretical and practical results, novelty: mechanisms of the influence of thiamine and its metabolits on activity of cathepsins have been first analyzed. The used methods are implemented in the large special workshop on biochemistry at the biological faculty of I.I. Mechnikov Odesa National University.