Kulieshova D. Oxidative stress and the state of enzymatic systems of protection against free-radical damage in adolescents with obesity

Object of study - oxidative stress and enzymatic protection from its damaging effects (antioxidant enzyme system and disposal system cytotoxic carbonyl products of free radical oxidation). The purpose and objectives of the study. To deepen the understanding of the mechanisms of formation of obesity during puberty to work is the study of free radical oxidation of proteins, lipids, and the state of the enzyme systems protect cells from free radical damage in adolescents with obesity and insulin resistance in the background without it. Methods: spectrophotometry , fluorometer, radioimmunoassay ,linked immunosorbent assay, аnalytical electrophoresis, electrochemical biosensor analysis, the statistical analysis. Theoretical and practical results, novelty: the body adolescent obesity appears oxidative stress is more pronounced in early than in late puberty. Its manifestations are enhanced by the development of obesity on the background of insulin resistance. One of the causes of oxidative stress in adolescents of both age groups studied obese against insulin resistance, as well as in patients 16 - 18 years of age with obesity without insulin resistance is due to increased giperkateholaminemiey prooxidant effects on the body. An additional factor in the formation of oxidative stress in late adolescence adolescent obesity on the background of insulin resistance, is the reduction of the power of the antioxidant protection at the expense of the implementation of adaptive mechanisms for its endocrine regulation. Field of application and performance: research of pathogenesis of obesity, lecture course of biochemistry and pathology.