Stasyuk N. Sensor systems based on arginase I, conjugated with micro- and nanopсаrriers

An arginine-containing affinity sorbents based on mineral matrixes were presented; the simple scheme of isolation and chromatographic purification of arginase I on the arginine-macroporius silochrome sorbent was proposed. The original method of covalent immobilization of arginase I on the surface of metalic micro- and nanocarriers have been developed at first and some physico-chemical characteristics of biofunctionalized materials were investigated. An recombinant human liver arginase I was used at the first for the construction of arginine-selective sensor systems. Developed sensor systems were applied to the samples of commercial pharmaceuticals for arginine testing and it was shown that the estimated contents are in a good correlation with that declared by producers and the results of referent chemical method.