Bilous S. Culture of Populus tremula L. (DNA-identification, morphogenesis in vitro, adaptation in vivo)

Українська версія

Thesis for the degree of Candidate of Sciences (CSc)

State registration number

0413U005575

Applicant for

Specialization

  • 06.03.01 - Лісові культури, селекція, насінництво

08-10-2013

Specialized Academic Board

Д 26.004.09

National University of Life and Environmental Sciences of Ukraine

Essay

The research of micropropagation of aspen different morphological forms were done. Established that ways of morphogenetic potential tissue explants of Populus tremula L. by in vitro selection, modification of nutrient components and cultivation conditions at different stages of morphogenesis. The processes of callus induction and indirect morphogenesis for different types of explants were research. Using cytological and anatamo-histochemical analysis the effect of exogenous hormonal factors on the differentiation of cells and indirect processes of morphogenesis were determined. The basic conditions for induction the process of root formation of Populus tremula L. were determined. Found most effective adaptation of plants in vitro in a greenhouse, thus ensuring 100% engraftment adapted plants in the environmental conditions.

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