Levkovich S. Lipid peroxidation and antioxidant system in the body is silver carp (Hypophthalmichthus molitrix Val.) for Сadmium and Lead intoxication

Object of investigation. The system of antioxidant protection of silver carp body is the object of investigation. Aim of investigation. Clarifying of the mechanism of toxic influence of high concentrations of cadmium and plumbum in water on system state of antioxidant protection, as well as on oxygen-transport function of blood and some links of metabolism in silver carp body (Hypophthalmichthus molitrix Val.) is the aim of investigation. Methods of investigation. Such methods of investigation as biochemical (spectrum and photometrical, chromatographic), statistical (method of variation statistic with the use of Student's t-criterion) were used. Novelty. Firstly it is determined tissue specific in enzyme reflection of antioxidant protection (superoxide dismutase, catalases, glutathione peroxides and glutathione reductase) and formation of lipid peroxidation products in silver carp body because of the effect of 1 MPC and 5 MPC of cadmium and plumbum. It is revealed contents decreasing of free sulhydrated groups and contents increasing of oxidative modifications of protein content in all investigated organs and tissues of fish. It is determined methaemoglobin increasing due to active increase of methaemoglobin reductase in fish erythrocytes. It is firstly highlighted that under the influence of cadmium and plumbum decreasing of polyunsaturated fatty acids contents in erythrocytes, liver and kidneys of silver carp took place. Results of investigation. The work is dedicated to the study of the mechanism of toxic influence of high concentrations of cadmium and plumbum on system state of antioxidant protection, as well as on oxygen-transport function of blood and some links of metabolism in silver carp body. The influence of cadmium and plumbum ( 0.1 and 0.5 mg/L of plumbum; 0.005 and 0.025 mg/L of cadmium) on processes level of antioxidant protection in tissues of liver, gills, muscles, kidneys and erythrocytes of fish is investigated. It is determined the high sensitivity and tissue specific in enzyme reflection of antioxidant protection (superoxide dismutase, catalases, glutathione peroxides and glutathione reductase) and formation of lipid peroxidation products ( TBA-active products and lipid hydro peroxides) in silver carp body because of cadmium and plumbum effect.