Vakal S. Biochemical mechanisms of rat pancreas damage upon long-term suppression of gastric acid secretion

The thesis deals with the study of dysbiotic-inflammatory pathway role in structural and functional damage of pancreatic gland upon long-term suppression of gastric acid secretion. The following parameters were determined in the study: histological and cytomorphological state of rat pancreatic tissue, intensity of free-radical processes, indices of pancreatic antioxidative system state, inflammation level, activity of pancreatic hydrolases in blood serum and pancreatic tissue, proteolytic profile of pancreatic juice, expression level of few genes involved in pancreatitis development, regeneration and hypertrophy upon dysbiosis and normobiosis in rats with chronically suppressed gastric acid secretion. Results of performed investigations clearly indicate that following biochemical mechanisms are involved in pancreatic damage upon these experimental conditions: violation of prooxidative/antioxidative balance, changes of cytokine profile, intrapancreatic activation of hydrolytic enzymes, and shift in expression pattern of few studied genes in pancreatic cells. The leading role of pathogenic microbiota in violation of structural and functional state of rat pancreas upon these conditions were established.