Prystupa O. Biochemical methods of diagnosis and monitoring the treatment of animals suffering from fatty hepatodystrophy.

Object is a fatty hepatodystrophy in rats and cows. Purpose - to establish early diagnosis informative biochemical indicators and develop effective treatment for animals, that suffer from fatty hepatodystrophy. Methods: clinical (research of body temperature, pulse rate, number of respiratory movements and rumen reduction), biochemical (determination of blood parameters characterizing protein synthesizing, urea creating, bile producing, biliary, carbohydrate, lipid, liver functions, and activity indicator for liver enzymes histology (performed histological analysis of liver samples) and statistical (biometric results processing). Novelty: by studying pathogenic mechanisms of fatty liver dystrophy in rats and cows biochemical methods of diagnosis and treatment monitoring of patients with hepatodystrophy are offered. For the first time after hepatodystrophy toxicity was modeled in rats, we investigated the therapeutic efficacy "Hepalen" (Patent for useful model № 73865 10 . 10. 2012). Levels of bile acids in the blood of high-yielding cows suffering from fat hepatodystrophy were studied, cholate changes during the development of the pathology and treatment are shown. Protein synthesizing, urea creating, bile producing and excreting and liver functions in dairy cows were investigated, which helped to establish highly informative early tests for the diagnosis of fatty hepatodystrophy and monitoring treatment of sick cows. Content of lipid peroxidation products (lipid hydroperoxides, TBA- active products) and activity of antioxidant defense enzymes (superoxide dismutase, glutathione peroxidase) in cows, that suffer from fatty hepatodystrophy were studied, this is important determination of liver pathologies. For the first time the efficiency of the drug "Hepalen" for treatment of cows suffering from fat hepatodystrophy is studied. Influence of "Hepalen" on the functional state of the liver structure and processes of lipid peroxidation and activity of antioxidant defense enzymes are determined. Results: we developed, tested experimentally and theoretically proved informative laboratory biochemical tests for early diagnosis and control of patients with fatty hepatodystrophy high-yielding cows treatment. Liposomal drug "Hepalen" for cows suffering from fat hepatodystrophy (patent number 73865 Therapeutic drug " Hepalen ") treatment was developed and tested.