Stepanets I. Potential protein markers of chronic alcohol intoxication.

Biochemical parameters were investigated in blood serum of rats: activity of gamma-glutamyl transpeptidase, alanine aminotransferase, aspartate aminotransferase, alfa- amylase, total bilirubin, low density lipoproteins, high density lipoproteins and metal cations content (Cu2+, Fe2+, Zn2 +) in liver homogenate of rats under alcohol intoxication development. Changes in protein fractions of brain, liver and blood serum of intoxicated animals were shown. Increase of G-class antibodies content in the blood plasma by 32% on the 11th day of alcohol consumption was stated. It is proved that chronic alcohol intoxication is being accompanied by changes in the antigenic composition of blood serum of rats. Antibodies of blood serum of rats with chronic alcohol intoxication are characterized by 40% lower affinity to the antigen of control serum than to antigens of native serum. Chronic alcohol intoxication caused the formation of autoantibodies to antigens. Under chronic alcohol intoxication, content of autoantibodies to antigens in blood serum increases - exceeding the norm in 26 times - to albumin and in 9 times - to insulin. The content of glycoproteins in blood serum of rats was increased on the 1, 3, 7, 11 and 21-th day in 1,06; 1,09; 1,23; 1,44 and 1,07 times, compared with the control level under chronic alcohol intoxication. Chronic alcohol intoxication is accompanied by the circulation of proteins with abnormal structure - glycated hemoglobin and forms of carbohydrate deficient transferrine. The increase of glycated hemoglobin content in 2; 2,3; 3,5 and 1,9 times on the 3, 7, 11, 21th day, compared with the control is shown. Increase of monosialo - , dysialotransferrin content by 10%, 16-18% on 11-th day is stated. The identification of carbohydrate deficient transferrine and glycated hemoglobin forms in blood serum can also serve as marker.