Mishchenko T. Ecological assessment of herbicides water pollution by metabolic indexes of carp (Cyprinus carpio)

The dissertation thesis is devoted to the search and selection of the most effective indicators for ecological assessment contamination herbicides pollution of water reservoirs by glyphosat contained herbicides among lipid metabolism, lipid peroxidation, activity of some enzymes of carp (alkaline phosphatase, holinesterase, ?-hlutamiltransferaze). Found that carp of different ages specifically changes lipid metabolism in response to investigated toxicants. Two years old carps were the most stable group of fish with stable indexes. Underyearlings was experiencing greater influence, evident in the accumulation of lipids in fat depots and accompanied by inhibition of lipase. For fry was seen enhance of lipolysis. Research of lipid peroxidation carp underyearlings showed no significant activation of lipid peroxidation as a final product accumulation in tissues does not occur. By correlation, variance and regression analysis identified the most effective biomarkers for monitoring water among metabolic parameters defined in carp, and on this basis were development mathematics' models of determination glyphosat concentration in water. Offered the use of correlation coefficient GGT activity in the liver and serum. Done multiple regression analysis of indicators lipid peroxidation, on the basis of which presented three models of linear regression. The verification of mathematical models done in vivo, proved its validity and the possibility of forecasting concentrations of roundup in water by the biochemical parameters of carp. The assessment of water pollution of Chernihiv region by glyphosat contained herbicides using mathematical models was done, established excess MAC, as evidenced by hydrochemical analysis, is accompanied by reserve antioxidant status of carp and significant fluctuations in enzyme activity (indicators of stress reactions). The most significant protective effect of BPS-44 appears on the level of lipid metabolism of underyearlings.