Khopta N. Effect of Cadmium Salts and Nitrites on Metabolism in Bone Tissue.

The object – metabolic process of biological elemental composition and structure of bone tissues of rats under the toxic lesson of just cadmium ions and nitrates ions and their combinations. The purpose is clarify the characteristics of metabolic processes, bioelment composition and structure of the bone tissues under conditions of separate and combined effect of cadmium chloride and sodium nitrite on the basis to justify the corection of the violations. Methodss:complex biochemical (spectrophotometry, titrimetric and ELISA), biophysical (atomic absorption spectrophotometry), histological and radiological (densitimetry) methods, statistical method (variation statistics using Student t-test, correlation analysis). This was discovered for the first time in complex research of biochemical and structural changes in the tissue of rats of combined and separated action of cadmium salts nitrites. On the basis of these, researches shows that under the influence of cadmium ions and nitrites result to dysmicroelementoses which is accompanied by violation of organic and mineral matrix of bone tissue as confirmed by significant changes in the level of hydroxyproline as markers of colagen, as calcium, magnesium, zinc, copper, acid and base phosphatases activity. New data shows that the toxicity of cadmium ions and nitrates results to lower bone mineral density, violation of calcitonin and parathyroid hormone. Histological researches confirmed that femurs metabolism is accompanied by disorganisation of collagen fibers and orderly placement of bony plates. For the first time, this experimental model shows that the use artichoke extract accelerates the development of compensatory restorative processes in the bone tissue.