Danchenko N. Taurine and glycine influence on bile acid and lipid compounds of rat's bile in condition of diabetes

The present study was focused on research of aminoacids glycine and taurine influence on bile acids and lipid composition of bile in condition of diabetes mellitus. As a result of experimental research it was shown that exogenous taurine introduction (3 mg / 100 g of animal body weight) to healthy rats significantly intensified work of liver polyenzyme systems, providing taurine conjugation with bile acids. However, in this condition conjugation with glycine decreased, that was confirmed additionally by substantial changes in glyco - to taurocholates ratio in bile. Glycine in lower tested dose (4,9 mg / 100 g of animal body weight) more substantially contributed to intensification of bile acids conjugation processes in healthy rat liver not only with this amino acid, but also with taurine, that accompanies with considerable increase of conjugation coefficient. In alloxan-induced model of diabetes mellitus decrease of effectiveness of liver polyenzyme systems, which provide bile acids and individual lipid fractions biosynthesis was observed. Primary changes of polyenzymes systems activity of bile acids synthesis determine changes in functional properties of bile, that was confirmed by authentic changes in conjugation and hydroxylation coefficient and glyco - to tauroconjugated acids ratio. Glycine increased fatty acids content and normalized triglycerides and cholesterol ethers ones in diabetic rats. Taurine normalizes fatty acids content, significantly increases phospholipids and free cholesterol level, simultaneously significantly decreases cholesterol ethers level. Both investigated amino acids normalized glucose level and protein metabolism in diabetes mellitus condition; caused stabilizing influence on lipid composition of rats blood. However, glycine more positively affected phosphorus and iron level, but taurine - magnesium.