Bodnar Y. Oxidative processes activity and the synthesys of hormones by cow ovarian granulose cells.

The thesis is devoted to the study of respiratory activity and reduction abillity, activity and izozyme content of lactate and malate dehydrogenases and antioxidant enzymes, proteins (soluble and structural) in granulose cell layer of cow follicles and their connections with the ability of granulose culture to form steroid hormones. Comprehensive research showed features of redox processes in granulose cell layer of cow follicles, course of which depends on the physiological state of ovarian follicle and size, from which they are derived. It is proved that cells in vitro can form steroid hormones, intensity of steroidogenesis depends on the respiratory activity, reduction abillity, lactate and malate dehydrogenase izozymes activity and content in granulose. Features of free radical oxidation, the activity of antioxidant enzymes and izozyme content, formation of sex hormones in granulose were detected. The changes of protein content (soluble and structural) in cell culture and correlation between its indexes and the ability to form sex hormones by granulosa cells were determined. It was been proved that the intensity of oxidative processes and the synthesis of hormones cells depend on the length of cultivation, the number of passages in culture and substrates in medium. The results of experimental studies have provided material for obtaining a veterinary drug that has 2-5 times higher concentrations of sex hormones than in primary cell culture. On the basis of the culture medium a drug was manufactured to stimulate the reproductive function of cows. The drug incorporates the culture medium containing the granulose cells of ovarian follicles cows in the form of liposomal emulsions.