Zaitseva O. The features of oxidative-nitrosative stress development and their correction at experimental osteoporosis

Object: processes of oxidative-nitrosative stress, the state of mineral metabolism and bone tissue at alimentary osteoporosis and enhanced nitric (II) oxide production induced by BCG vaccine injection. Aim: to determine the level of oxidative processes development, mineral metabolism and bone tissue condition at alimentary osteoporosis in rats caused by vitamin D3 deficiency and effect of BCG vaccine as inductor of enhanced NO endogenous production on the course of detected disturbances. Methods: biochemical (spectrophotometry, chromatography, radioisotope analysis and so forth), physico-chemical (X-ray, EPR spectroscopy), osteometric, immunological (ELISA, Western blot analysis), methods of light microscopy and flow cytometry, statistical methods. It was shown that vitamin D3 insufficiency leads to significant disturbances in the skeletal structure of the rats and the development of alimentary osteoporosis. At the same time alimentary osteoporosis accompanied with the increasing of protein oxidative modifications, lipid peroxidation and reducing of antioxidant defense. Disturbances in the mineral metabolism and structure of the extracellular matrix in particular the decreasing of mineral and organic components stability in bone tissue at alimentary osteoporosis were also defined. BCG vaccine caused the significant activation of the endogenous nitric oxide synthesis but didn't affect the redox processes which take place in rats with alimentary osteoporosis. Additional BCG vaccine injection led to the collagen stabilization, keeping mineral components in bone and decrease of resorption processes. Obtained results indicate a possibility of partial BCG correction some processes in bone tissue which take place at alimentary vitamin D3-deficient osteoporosis