Shalovylo Y. The molecular-genetic basis stability of Scots pine (Pinus sylvestris L.) to bacterial diseases

Object of research - healthy and infected Scots pine of different ages and pine stands affected by bacterial diseases. Aim - to isolate and identify the causative agents of bacterial diseases of Scots pine, reveal their pathogenic properties and study the features of a defensive reaction against bacterial pathogens of Pinus sylvestris L. Research methods - analytical and biochemical, genetic engineering, microbiological, molecular biology, phytopathological, statistics. Scientific novelty - were identified the pathogenic properties of isolates of Bacillus pumilus strains to Scots pine and founded that they can cause soft rot in seedlings of different ages. For the first time were isolated proteins with low molecular weight - defensins from seeds of Scots pine, which having antimicrobial activity. Were identified two new sequences of defensins genes: PsDef5.1 and PsDef5.2, which deposited in the GenBank NCBI database under accession numbers KJ601733.1 and KJ601734.1. The expression levels of those two genes PsDef5.1 and PsDef5.2 were studied in different organs on different stages of Scots pine development. The P. abietaniphila had attributed to the group of PGPR-bacteria, because this strain stimulated the growth of Pinus sylvestris L. seedlings and inhibited the growth of the fungus Fusarium sp. Practical value - a method of molecular-genetic identification of phytopathogenic bacterium B. pumillus in the natural pine forest stands was developed, it allows to identify the pathogen in the pine tissues on early stage of pathogenic process. Was proposed use of P. abietaniphila strain in greenhouse for suppression of fungal pathogen Fusarium sp. Area of application: forestry.