Lazarenko-Chernousova I. Protein and amino acid rats’ blood composition when affected by lead of different dispersion

The thesis is aimed at defining the protein and amino acid rats’ blood composition when affected by lead of different dispersion. A biological model of rats’ poisoning with plumbic acetate and lead nanoparticles was developed as well as protein and amino acid rats’ blood composition when affected by lead of different dispersion was studied. It should be noted that rats’ poisoning with plumbic acetate and lead nanoparticles increases the amount of lead in blood, liver, kidneys, lungs, heart, spleen and brain. Use of drug «Glutargin» reduces the content of the toxic agent in tissues and organs of rats poisoned with lead of different dispersion forms. The increase of serum aspartate amino transferase, alanine aminotransferase, lactate dehydrogenase, gamma-glutamyltransferase and alkaline phosphatase activity in the blood of rats poisoned with plumbic acetate and lead nanoparticles was found. It wasrevealed that vaccinating rats with plumbic acetate and lead nanoparticles causes reduction of albumins, alfa1- and alfa2-globulins content and increases the level of beta- and gamma-globulins in blood and also leads to the increase of Ig G1, Ig G2 level, caeruloplasmin, transferrin with molecular weight of 85–80 and 75 kDa, prealbumins with molecular weight of 60–50 kDa and reduces the content of prealbumins with a molecular weight of 20–15 and 45 kDa. It was found that plumbic acetate favors the accumulation of high- and low-molecular circulating immune complexes in blood, whereas lead nanoparticles cause the reduction of high-molecular circulating immune complexes in blood. It was noted that under the influence of various dispersion forms of lead, significant changes of free amino acid content in rats’ blood serum are observed. The corrective action of «Glutargin» in reducing the accumulation of lead in body and content stability of free amino acids, the level of circulating immune complexes and blood protein fractions of rats poisoned with different dispersion forms of lead was found.