Kushkevych M. Ontogenetic changes of localization, level of cellular prion and its relationship with ATPases activities

The ontogenetic changes of localization and level of cellular prion was studied and a correlation with the activities of Na+-K+- and Ca2+-ATPases in prion replication (medulla oblongata, cerebellum, spleen, jejunum) and other (liver, kidneys, muscle) rats' tissues was calculated. These data are valuable for the study of PrPC functions in the body and pathogenetic processes of prion diseases. The PrPC localization in the medulla oblongata, cerebellum and peripheral prion replication and other organs of rats was determined. The total level of PrPC and molecular isoforms (including di-, mono- and nonglycosylated) increased in 6 months animals compared to 1 month rats, and decreased in 30 months animals compared to 6 months. The decrease in activity and changes of the kinetic parameters of Na+-K+-, Ca2+-ATPases was showed in the experiments with increasing of animals' age. It indicates the reduction of ATP hydrolysis intensity and the increase of enzymes affinity to substrate (ATP). The levels of sodium and potassium ions were not significantly changed but the total calcium level was increased. The factors of ages and tissue localization had different effects on ontogenetic dynamics of PrPC, Na+-K+- and Ca2+-ATPases. To predict of the pathology, the models of age related changes of the studied indicators were created.