Chepets O. Pathomorphological and molecular genetic characterisation of invasive metastatic features of endometrioid adenocarcinoma of uterus.

In the current dissertation based on the results of the pathomorphological and molecular genetic studies, it was revealed that invasive endometrioid adenocarcinoma of the uterine corpus is characterized by the lower expression levels of estrogen receptors-а, progesterone receptors, в-catenin, E-cadherin, caspase-3 in the tumour glandular cells, the lower p16 expression level in the tumor stromal cells and the reduced mRNA expression level of CDH1 gene compared with proliferative endometrium. The invasive endometrioid adenocarcinoma of the uterine corpus is characterized by the increased expression levels of p53, p16, Кі-67, MMP-9, TIMP-1, VEGF, VEGFR-2, the higher transcriptional activity of VEGF and CTNNB1 genes compared with proliferative endometrium.Invasive adenocarcinoma is characterized by the higher expression levels of Ki-67, р53 and caspase-3 in the glandular tumor cells compared to non-invasive endometrioid adenocarcinoma. In the glandular tumor cells of the central area of invasive endometrioid adenocarcinoma of the uterine corpus the expression levels of Ki-67 and caspase-3 are significantly higher than the same levels in the area of tumor invasion in the myometrium. The metastatic endometrioid adenocarcinoma of the uterine corpus is characterized by the higher medians of mRNA expression levels of VEGFA and CTNNB1 genes, significantly higher expression levels of estrogene receptors-а and VEGFR-2 in tumor cells and reduced expression levels of progesterone receptors, MMP-9, TIMP-1, в-catenin, compared to non-metastatic endometrioid adenocarcinoma. There is a trend to decreasing of the mRNA expression level of the CDH1 gene and E-cadherin expression level in metastatic enomerioid adenocarcinoma compared to non-metastatic tumour.