Voieikova D. Structural and functional state of the inner mitochondrial membrane rat hepatocytes under conditions of steatohepatosis

In this paper lipid and phospholipid composition of the mitochondrial membrane was determine under conditions of diet- and glutamate-induced steatohepatosis; enzymatic activity of respiratory chain complexes was assessed in inner mitochondrial membrane; protein content in hepatocytes according molecular weight was determined and changes in biochemical markers of serum was found in conditions of steatohepatosis caused by different etiological factors. In addition, it all mentioned parameters were assessed in animals with glutamate-induced steatohepatosis which as correction treated pioglitazone. Steatohepatosis was shown to be associated with development of pathological changes in the inner mitochondrial membrane, which further show symptoms of oxidative stress. The significant increase in fat mass and changes in biochemical parameters of blood serum were demonstrated on both models. Triglycerides and cholesterol were increased in rat's serum under both methods of modeling. Significant increase in cholesterol and oxidized forms of major phospholipid was found in mitochondrial membrane in diet-induced and glutamate induced steatohepatosis. The development of diet-induced steatohepatosis results in increase cholesterol content by 2,4 times (p<0,05), and the development of glutamate-induced steatohepatosis results in increase by 3,5 times (p<0,001). It was found that the development of diet- and glutamate-induced steatohepatosis results in a significant increase oxidized phospholipid forms. The content of lizophosphatidylholin and lizophosphatidyletanolamin increased by 1,7 (p <0,01) and 1,4 (p <0,01) times under conditions of high caloric diet and by 9,2 (p <0,001) and 5,5 (p <0,001) under glutamate-induced steatohepatosis, which may indicate about reduction of ATP in hepatocytes.