Rosalovsky V. Disorders of prooxidant-antioxidant balance and functional state of rat blood under condition of chlorpyrifos intoxication, and their treatment with vitamins A and E complex.

This study is dedicated to the effects, which acute exposure to different doses of chlorpyrifos (CPF) causes on the prooxidant-antioxidant status and on physico-chemical and functional properties of the rat peripheral blood components. The results show that mechanisms of CPF toxicity are not limited only to its anticholinesterase effect, but are also related to disturbance of the RedOx homeostasis, in particular, by oxidative stress induction and changes in morpho-functional characteristics of the blood cells. We also demonstrate the possibility to use the A and E vitamins complex as an antioxidant factor for correction of hematotoxic effects, and for prevention and mitigation of the prooxidant processes development in the rat blood after CPF exposure. Based on the obtained experimental results we substantiated a method for reducing chlorpyrifos-induced prooxidant activity and correcting hematotoxic effects (studied in peripheral blood of CPF-intoxicated rats) with a complex of vitamins A and E. Moreover, it has been established that individual biochemical parameters of the LPO processes, blood antioxidant system, and certain physicochemical parameters of red blood cells (resistance to acid hemolysis, content of ligand forms of hemoglobin and its affinity to oxygen) can be used as additional diagnostic features of CPF intoxication.