Savchuk T. Regenerative processes in experimentally damaged bone tissue of rabbits and the influence of stem cells on their stimulation

The thesis presents a generalization and a new solution of the scientific task concerning the study of the peculiarities of the processes of restoration of experimentally damaged bone tissue due to the influence of allogeneic mesenchymal stem cells introduced into the organism of recipient animals in various ways. The results of macroscopic studies, x-ray, histological and biochemical changes (the content of Calcium, Phosphorus and alkaline phosphatase activity) in the blood serum of rabbits in experimental mechanical damage to bone tissue and the influence of transplanted allogeneic mesenchymal stem cells in the jugular vein and directly into to the place damage. It was found that mechanical damage to bone tissue causes a pronounced reaction from the bone tissue, adjacent soft tissues and the overall reaction of the body. During the introduction of allogeneic mesenchymal stem cells, activation and acceleration of all phases of reparative osteogenesis is observed, starting from the 3 day, where these conditions of defect restoration are almost completed on the 28 day of the study, while in animals of the control group on the 42 day. Comparison of the effectiveness of two methods of using allogeneic mesenchymal stem cells for reparative osteogenesis showed that the introduction of allogeneic mesenchymal stem cells directly into the place of experimental bone tissue damage, the recovery processes at all phases of reparative regeneration are more pronounced than after the introduction into the jugular vein, which is of great importance for the consolidation of bone defect tissue, namely on the 3 day of research.