The thesis represents the results of researches of the peculiarities of clinical manifestation, blood parameters, pathomorphological, histochemical and immunohistochemical indices of the internal organs of piglets because of escherichiosis due to excess of Kuprum, Ferum and Cobalt in feed. Chemical and toxicological research has established an excess of the maximum allowable level in feed, which caused a significant increase of its content in the internal organs of clinically healthy and suffering from colibacteriosis and colienterotoxemia of piglets.
During the simultaneous influence on the body of piglets of E. сoli toxins and excess micronutrients, there were established a pathological leukocytosis, blood glucose levels decrease in hemoglobin and erythrocytes, in blood serum was increase in the content of total protein and relative content of γ-globulins, creatinine, total bilirubin, AsAT and AlAT, LF and α-amylase.
The excessive intake of Kuprum, Ferum and Cobalt in the body of clinically healthy pigs caused a decrease in the immune reactivity of the organism, as evidenced by a decrease in the area of the lymph nodes in the intestinal mucosa, in the lymph nodes of the intestine and the spleen were swelling and mucoid edema of the stroma, hypoplasia of the lymphoid tissue, a decrease of the area and weak division of lymph nodes into zones.
The features of the intestines of piglets were catarrhal enteritis and colitis, which were characterized by inflammatory mucoid and fibrinoid edema, and hyperemia of the intestinal membranes and their leukocyte infiltration, destruction of villi, an increase in the number of cell-like cells and their secretion. Immunosuppressive effects of E. сoli toxins and excess micronutrients in the lymph nodes of the intestine manifested by edema of the stroma, hyperemia, diffuse hemorrhages, and decreased area of the lymph nodes.
The features of the spleen were edema of the capsule and trabeculae, subcapsular hemorrhages, perivascular edema of blood vessels, lack of clear separation of the pulp on the white and red, diffuse infiltration of the red pulp by neutrophilic leukocytes and lymphocytes, reduction of the area of the lymph nodes and the width of the periorarterial vagina. In the kidneys, hypertrophy, serous glomerulonephritis and fibrinoid necrosis of the vascular glomeruli, granular and hydroponic dystrophy with necrosis of the epitheliocytes of the convoluted tubules, in which the lumen was detected by hyaline cylinders and erythrocytes, desquamation of the nephrocytes of the distal tubule sections, edema and focal infiltration with leukocytes and macrophages of the interstitial tissue were established.
