Romanovych M. The state of antioxidant system and immunobiological reactivity of broiler chickens under effect of probiotic drugs

The dissertation deals with study of the peculiarities of lipid peroxidation, oxidative modification of proteins, the activity of the antioxidant system, the hematological profile, the natural resistance and immunobiological reactivity of chicken broilers during the period of their cultivation under use of probiotic drugs. The research was carried out in 4 groups of broiler chickens: Control group was fed the standard fodder; Experimental group 1 received a probiotic BPS-44, made of strain of bacteria Bacillus subtilis ssp. subtilis 44-p in dose 0.21 g / kg; Experimental group 2 – respectively 1% Saccharomyces cerévisiae yeast; Experimental group 3 – respectively 2% Saccharomyces cerévisiae yeast. As a result of our study it was proved that introduction of probiotic preparations with different action leads to changes in the hematological profile, increase in protein content and activity of enzymes of AOS, decrease in the intensity of products of LPO and OMP, increase in parameters of cellular and humoral immune response. Thus, application to chickens of experimental groups of the preparation BPS-44 and 1% and 2% of Saccharomyces cerevisiae yeast increased the oxygen-transport and immune potential of the blood and the protein synthetic function of the liver. It has been established that feeding chickens with the preparation BPS-44 and Saccharomyces cerevisiae yeast reduced the content of the intermediate and final products of the LPO (p<0.01-0.001) in the blood, as well as the aldehyde and ketone derivatives of the oxidative modification of proteins, especially in the 41-days age under action of 2% yeast Saccharomyces cerévisiae (p<0.05). At the same time it was shown that the probiotic drugs caused an increase in the enzymatic level of the antioxidant system in chickens. The stimulatory effect of the probiotic drugs on cellular and humoral links of non-specific resistance of broilers has been demonstrated by higher indices of phagocytosis of blood pseudo-eosinophils (p<0.05-0.001) as well as bactericidal and lysozyme activity of serum (p<0.05-0.01) and the content of circulating immune complexes (p<0.05). The positive effect of BPS-44 and 1% and 2% Saccharomyces cerévisiae yeast on the formation of post-vaccine immunity was shown. Thus, the average titers of specific antibodies to Gumboro disease virus in chickens in experimental groups were higher at all trial periods (p<0.05-0.001). The stimulatory influence of probiotic drugs on the state of T- and B-cell immunity of poultry was established. In particular, in the blood of chickens in experimental groups, a greater number of T-lymphocytes (common, active and theophylline-resistant) and B-lymphocytes were detected and their functional activity increased due to the redistribution of the receptor apparatus of immuno-competent cells (p<0.05-0.001). An increase of number of T-active rosette-forming lymphocytes in the blood of chickens of experimental groups occurred under a decrease (p<0.05-0.001) of "zero", inactive in the functional relation T- and B-lymphocytes, and growth (p<0.05-0.001) of subpopulations with low and medium receptor density. The results of our research theoretically and experimentally substantiated the possibility of using of immuno-modulator drugs –Saccharomyces yeast and BPS-44 for vaccination of broiler chickens against Gumboro disease in order to increase the tension of post-vaccine immunity and antioxidant potential. In practical sense it can be concluded the expediency of the use of BPS-44 and Saccharomyces cerеvisiae yeast for increasing immuno-biological reactivity, performance and productivity of chicken broilers.