Object: genes expression level of VEGF, VEGF-A-189, PDGFC, FGF1, FGF2, FGFR2, FGFRL1, E2F8, HIF1A, EPAS1, CTGF, MYLK, MEST, PLAU, PLAT, PLAUR, SERPINE1, CLEC3B, TPD52, ITGB1, ITGAM, DUSP1, DUSP4, DUSP6 ,DUSP22, PTEN, FAT1, PPDPF, SFRP4, CTHRC1, EGFL6, TLR2, TLR4, TNF, ADD3, BiP and ADM of human. Aim: investigate the level of gene expression encoding the key factors of angiogenesis, proliferation and thrombosis in subcutaneous adipose tissue of men with obesity and insulin resistance to clarify their role in the development of obesity and its metabolic complications, as well as to identify possible correlation between changes in the expression of these genes and the body mass index (BMI). Methods: cultivation of total RNA extraction, spectrophotometric determination of the nucleic acid concentration, cDNA synthesis by reverse transcription, quantitative polymerase chain reaction, electrophoretic analysis of amplification products, bioinformatic analysis, computer analysis of polymerase chain reaction results, statistical methods of date processing. For the first time, it was found that in adipose tissue of obese men the expression of genes encoding the major factors of angiogenesis (VEGF-A, VEGF-A-189, FGF-2, and FGFRL1) is suppressed, but the expression levels of FGF1, FGFR2, E2F8, HIF1A, PLAT, and CLEC3B genes is up-regulated. Furthermore, glucose intolerance in obesity is associated with increased levels of VEGF-A, FGF2, FGF1, E2F8, PLAU, PLAUR, and SERPINE1 gene expressions, and down-regulation of CLEC3B gene in subcutaneous fat tissue compared with obese patients with normal glucose tolerance. It has been shown that the level of DUSP1, DUSP4, DUSP6, DUSP22, and PTEN, TLR4, and ADD3 gene expressions in subcutaneous adipose tissue of obese men with normal glucose tolerance is reduced compared with control, but the development of insulin resistance is accompanied by up-regulation of the level of protein phosphatase gene expressions. It has also been established that in obese adipose tissue the expression level of FAT1, PPDPF, CTGF, MEST, MYLK, TPD52, ITGB1, ITGAM, CTHRC1, SFRP4, EGFL6, TLR2, TNF, and ADM genes is increased, but the development of insulin resistance is accompanied by down-regulation of the level CTGF, MYLK, TPD52, ITGB1, CTHRC1, EGFL6, and ADM gene expressions and up-regulation of TNF and SFRP4 genes. Extremely important moment of this work is the study of miRNAs whose binding sites were detected in the EGFL6, SERPINE1, PTEN, ITGAM, CTHRC1, TLR2, PLAU, and PLAUR mRNAs using a bioinformatics assay. The level of miR-7, miR-21, miR-143, miR-145 and miR-190b miR-143, miR-145 and miR-190b expression in the subcutaneous adipose tissue of men with obesity and normal glucose tolerance is reduced but miR-19a is increased. Since changes in the expression of miRNA and mRNA are inversely directed, this indicates the involvement of the investigated miRNAs in regulating the level of target mRNA expression at the post-transcriptional level. The obtained results point to the important role of changes in the expression of key regulatory genes in the development of obesity and its metabolic complications through genome reprogramming.
