Pavlova O. Key proteins of thiamine metabolism and functional state of nerve cells with different supply of organism with the vitamin В1

It has been found that the increase in THTR-1 content, which occurs at the slc19a2 gene expression level in all investigated brain regions under conditions of alimentary avitaminosis, cannot be offset by a single administration of a high thiamine dose. It has been established that under B1-avitaminosis condition there was both the enzyme activity and the protein decreases. It has been shown that under the conditions of chronic alcoholic intoxication there is a decrease in the TPK protein content in all studied brain sections and decreased enzyme activity in the brain. The increased astrocytes sensitivity to TD of varying degrees has been confirmed - even with limited thiamine admission, GFAP inhibition is observed. However, these changes are reversible, as evidenced by an increase in protein content caused by one-time administration of vitamin. Changes in the neurofilaments level are observed only under conditions of deep alimentary avitaminosis, and the decrease in protein content occurs only in cerebellum. However, the thiamine administration reduces these changes and even stimulates the increase in the NF content. These data demonstrate the priority of neurons in the distribution of thiamine in the brain. In case of chronic alcoholism, the normalization of the indicators was possible only with the co-administration of thiamine with the Metovitan drug. The renewal of neurospecific cytoskeleton structures suppressed under conditions of TD states is a basis for scientifically substantiated practical recommendations on the feasibility of using the proposed drug combination (a high thiamine dose and drug Metovitan) for the prevention and / or treatment of neurodegenerative pathologies induced or accompanied by thiamine deficiency.