Postovoitova A. The intron length polymorphism of actin genes as an effective tool for intraspecific and interspecific genetic differentiation of plants

A new approach for genotyping based on assessment of the intron length polymorphism of actin genes has been presented in the dissertation. The possibility of its use for DNA profiling and differentiation of plants at the specific, population, varietal and intravarietal levels has been studied. Based on the results of the bioinformation search and actin gene analysis in the genomes of monocotyledonous and dicotyledonous plants, the design and synthesis of universal PCR primers was carried out, using which intron length polymorphism of actin genes in representatives of the families Poaceae, Linaceae, Solanaceae and Brassicaceae was studied. In addition, by the example of flax intravarietal analysis the effectiveness of the approach for assess the intron length polymorphism of actin genes was confirmed in comparison with the popular SSR markers and TBP method. The same efficiency of DNA markers that detect the intron length polymorphism of actin genes compared to SSR markers and higher efficiency compared to the TBP method was established. In general, the data obtained indicate that a new approach for evaluation of the intron length polymorphism of actin genes is the informative tool for the plant molecular genetic analysis, which may find application in breeding and population-genetic studies.