Krasnoperova E. Serine-threonine protein kinases of the SnRK1 subfamily (KIN10 and KIN11) of Arabidopsis thaliana: peculiarities of functioning and participation in cell division

The structures of the catalytic subunits (KIN10 and KIN11) of SnRK1 protein kinase and human BRSK1 protein kinase were compared. The results of clusterization of the catalytic domains of all known A. thaliana protein kinases and the human BRSK1 protein kinase catalytic domain show, that KIN10 protein kinase is the closest plant homologue of human BRSK1. The intracellular localization of the KIN10-RFP chimeric protein in protoplasts of A. thaliana was shown. The levels of KIN10 expression in different organs of A. thaliana were analyzed. A high level of KIN10 transcripts in the aerial part of the plants was recorded. Correlation of KIN10 expression under conditions of salt, osmotic and energy stress after 2, 8 and 24 hours was demonstrated. Changes in the growth and development of the roots of seedlings of transgenic lines of A. thaliana with enhanced and suppressed expression of KIN10 under normal conditions and under conditions of energy deficiency were analyzed. Low levels of the mitotic index and fluorescence of γ-tubulin in the root cells of kin10 and kin11 knockout lines of A. thaliana under normal conditions and under conditions of energy deficiency were detected. It was shown that by potential phosphorylation of γ-tubulin on Ser-131, KIN10 protein kinase can affect the structure of the small γ-tubulin complex γTuSC and its further formation in the ring γ-tubulin complex γTuRC, thereby regulating cell division processes.