Kovpak O. Biological Properties of Stem Cells and their Effects on the Restoration of Animal Myocardium after Experimental Pale Infarction

The dissertation deals with the current issues of veterinary medicine, in particular, experimental solution of the scientific issue of the effect of stem cells derived from myocardium, bone marrow, and adipose tissue on the restoration of myocardium after experimental pale infarction. Study were focused on the myocardium-derived cultures of stem cells as an alternative source of cellular material to treat heart diseases. It is important to emphasize that in the course of in vitro cultivation, cells in the culture tended to change their phenotypic characteristics: morphology, expression of CD markers, and karyotype. Since the therapeutic effect of the studied cultures on the development of experimental myocardial infarction had been proven, we were to determine the best conditions for isolation from different sources and cultivation of cultures of cat stem cells for their further putting into veterinary practice. In addition, we were study the optimal concentration of insulin-like growth factor-1, growth factor of fibroblasts-2, growth hormone and Biolaminin 521 LN for proliferation index growth of stem cells of bone marrow, adipose tissue and myocardium, and accounted for the number of genetic errors in studied cultures.