Hrunyk N. The influence of structural and functional characteristics of lipid transfer protein on biological resistance of Pinus sylvestris L.

The object of study - seeds, embryos, seedlings, Scots pine trees under the influence of various environmental factors of biotic and abiotic origin. The research subject was the investigation of the expression of Scots pine LTP genes, obtaining recombinant protein, and examining its functional properties. Scots pine lipid transfer proteins genes have been identified. Coding sequences and intron-containing gene sequences of pine lipid transfer proteins were cloned, sequenced and deposited in the GenBank. Peculiarities of transcription of PsLTP1, PsLTP-B, PsLTP-D genes in Scots pine vegetative and generative organs and at different stages of plant ontogenesis have been studied. Changes in the expression levels of lipid transfer protein genes in juvenile pine plants have been shown under the influence of biotic, abiotic stressors, growth stimulants and heavy metals. Recombinant PsLTP1 was cloned and purified and further its functional characteristics were revealed, such as binding of hydrophobic molecules in vitro, inhibition of trypsin and insect pests amylase was investigated. The research results can serve as a basis for the use of genes of lipid transfer proteins as candidates for obtaining biologically stable genotypes of Scots pine. LTP genes can be used as genetic markers to detect salinity, osmotic stress and excess moisture genotypes of Scots pine. Field of application – forestry.