Konovalova O. Biochemical markers of functional state of rats’s liver under development of ordinary and cisplatin-resistant tumor and introduction of cytostatics

Dissertation is devoted to investigation of the biochemical markers of functional state of liver of tumor-bearing rats under development of ordinary and cisplatin - resistant Guerin's carcinoma and application of cisplatin, cluster compounds of rhenium and ferrocenes. It was compared the effect of cisplatin on the biochemical state of the liver of tumor-bearing rats at its application in liposomal form and in solution. It was shown that the usage of cisplatin in liposomal form didn't contribute to normalization of biochemical processes in the liver, i.e. inclusion of the drug in the lipid layer not almost reduced its hepatotoxicity. Biochemical markers of functional state of liver of tumor-bearing rats were studied under usage of rhenium-platinum antitumor systems based on rhenium cluster compounds with isobutyrate, indole and ferulate ligands administered in two ways: separately cisplatin solution and ten times rhenium compounds in liposomes, and in mixed liposomes, where both cytostatics were in the same liposome. Together with practically complete reduction of tumors, the application of the system on the first method leads to a decrease in the intensity of enzymes in blood, lipid peroxidation, cytolysis of hepatocytes, however, liver biochemical parameters do not reach normal. Аpplication of the rhenium-platinum system leads to a decrease in the activity of radical processes and increased activity of antioxidant enzymes, glutathione concentration and activity of glutathione protection enzymes in rat tissues with cisplatin carcinoma, that may explain the low level of enzyme and hepatocellular dystrophy in this group of animals. Also it was found that the usage of the of rhenium-platinum anti-tumor system tumor-bearing rats resistant carcinoma activates the gamma-glutamyl cycle.