Grytsenko M. Cytoskeletal and matrix protein genes expression in cultures of skin and lungs fibroblasts of rats in postnatal ontogeny

The dissertation is devoted to the structural and regulatory protein genes expression of the cytoskeleton and intercellular matrix, their products content and static mechanical stress influence on these parameters in primary cultures skin and lungs fibroblasts of Wistar rats 0.5; 1; 3 and 24 months age. It was established that the expression of Fgf1 and Fgf2 genes reached a maximum at a young age while Fgf8 expression was opposite that partially compensated changes in Fgf1 and Fgf2; the content of protein products in the lungs was much lower than in the skin. The ubiquitin gene expression and protein content measurements indicate that at a young age fibroblasts of the skin and lungs can protect connective tissue against damage induced by various external factors by enhancing the proteolytic destruction of damaged elements. It was found that the level of the Тp53 gene expression in ontogenesis was increased, but the accumulation of this gene protein has the opposite dynamic – was the highest in the cells of young rats. The level of β and γ actin genes expression and the content of their proteins was increased with age in both lungs and skin fibroblast cultures of animals. Estimation of the potential for connective tissue of the skin and lungs respond to mechanical stress was shown that both gene expression and the content of their proteins can respond to the action of this external factor. In the case of other genes, the quantitative characteristics of the response to mechanical deformation had no general focus. This demonstrated the multi-link pathways of the adaptive response of connective tissue cells to mechanical stress.