Muzyka L. The Level of Lipid Substances and Their Role in the Adaptation of Freshwater Mollusks to Environmental Factors of Various Nature

Українська версія

Thesis for the degree of Candidate of Sciences (CSc)

State registration number

0421U101807

Applicant for

Specialization

  • 03.00.17 - Гідробіологія

07-05-2021

Specialized Academic Board

Д 26.213.01

Institute of Hydrobiology of the National Academy of Sciences of Ukraine

Essay

The dissertation studies the consistent patterns of carotenoids, lipids of various classes and fatty acids levels in tissues and organs of freshwater mollusks according to the norm and under the influence of environmental factors of various nature. The scientific work examines β-carotene and xanthophyll level in the bodies of gastropod (Lymnaea stagnalis, Planorbarius purpura) and bivalve (Unio pictorum, Anodonta cygnea) mollusks. It determines that β-carotene and xanthophyll levels in the organs (tissues) of studied animals have species specificity. The paper clarifies the fact that filter-feeding mollusks – U. pictorum and A. cygnea – are characterized by lower indicators of both β-carotene and xanthophyll in the organs (tissues) than pulmonary mollusks L. stagnalis and P. purpura. The scientific paper analyses concentration time patterns of various poisonous substances effects on β-carotene and xanthophyll levels in tissues and organs of L.stagnalis, (namely local effect (Cd2+ Zn2+, Mn2+, Ni2+, Pb2+, Cu2+, Cr3+, Cr2O72- -ions), combined effect (phenol, ammonium chloride), and protoplasmic (urea) effect). It proves that carotenoids level in tissues (organs) of L.stagnalis under the action of ions depends on the time of exposition (2, 7, 14 and 21 day), the nature of affecting ion, which determines the peculiarities of its action, its concentration, and is characterized by tissue and organ specificity. The research determines the level of triacylglycerols, diacylglycerols, non-esterified fatty acids and phospholipids in the organs of L. stagnalis and U. pictorum. It accounts for the peculiarities of trematode infection effect on the lipids level in the body of L. Stagnalis and studies the level of triacylglycerols, diacylglycerols, non-esterified fatty acids and phospholipids according to bivalve mollusk sex. It also explains the distribution of lipid fractions due to tissue and organ specificity. The research paper analyses the quantity of saturated, monounsaturated and polyunsaturated fatty acids in the bodies of L. stagnalis and U.pictorum. It establishes that fatty acid composition of lipids of L.stagnalis and U.pictorum is represented by fatty acid with even and odd amount of carbon. It states that the peculiar feature of fatty acid profile of the two studied species is a significant level of branched fatty acids (iso - and anteiso - forms) as well as the presence of fatty acids with cis-configurations and trans-forms of double connections. The paper determines the effect of trematode infection of the level of fatty acids in L.stagnalis.

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