Solopova K. The state of antioxidant and immune systems in carp affected by aeromonosis and saprolegniosis, and their treatment.

The dissertation is devoted to the study of hematological profile, activity of immune and antioxidant defense systems in carp affected for aeromonosis and associated infection of aeromonosis with saprolegniosis, and as well as elucidation of the effect of antibacterial and symbiotic drugs on the activity of these systems in carp affected for aeromonosis. The experimental part of the work was performed in three stages. At the first stage of the dissertation, the intensity of lipid peroxidation processes, the activity of the immune and antioxidant systems in the body of carps, patients with aeromonosis and aeromonosis associated with saprolegniosis were clarified. It is shown that the defeat of carp by pathogens of aeromonosis associated with saprolegniosis leads to an increase in the content of products of LPO and OMP and a decrease in the activity of key enzymes of the antioxidant defense system. Under these conditions, an increase in the activity of humoral mechanisms of nonspecific resistance and immunosuppressive effect on the effector part of the immune response - a decrease in the number of T-lymphocytes (common and theophylline-sensitive) and a decrease in the functional activity of T-common lymphocytes . The purpose of the second stage of research was to determine the effect of the antibacterial drug «Flumek» alone and in combination with the seeds of milk thistle (Silybum marianum) on the intensity of lipid peroxidation and activity of enzymes of AOS, natural and adaptive immunity in healthy and sick aeromonosis carps. In order to study these issues, two experiments were conducted. At first experiment the effect of different doses of the antibacterial drug «Flumek» on the hematological profile, the state of the antioxidant defense system and the immune potential in clinically healthy two-year-old carp was investigated. The aim of the second experiment was to determine the effect of the antibacterial drug "Flumek" alone and in combination with the seeds of milk thistle (Silybum marianum) on the intensity of LPS and activity of AOS enzymes, hematological parameters, natural and adaptive immunity in healthy and patients with aeromonosis carps. The results of the study of antimicrobial susceptibility of Aeromonas hydrophila strains isolated from affected aeromonosis carp showed the high level of susceptibility of aeromonads to fluoroquinolone antibiotics, including flumequine, the active substance of the antibacterial drug «Flumek». The dose-dependent effect of the antibacterial drug «Flumek» on the biochemical profile, activity of the immune and antioxidant systems in both healthy fish and those affected by the pathogen of aeromonosis has been established. Its independent effect and combined action with the seeds of milk thistle on the body of sick fish has been clarified and treatment regimens have been proposed. The purpose of the third stage of research was to determine the effect of the symbiotic drug «Enteronormin», activated by an aqueous solution of iodine and selenium, on the state of immune and antioxidant protection in carp affected with aeromonosis. Its rehabilitative effect on the intensity of the processes of LPO and OMP, the expression of antioxidant enzymes, the activity of natural and adaptive immunity was shown.