Palytsia L. Biochemical mechanisms of the effect of fullerene C60 nanoparticles on toluene toxicity in different biological systems

The biochemical mechanisms that determine the synergistic effect of toxicity of carbon nanoparticles fullerenes C60 and the known chemical toxicant toluene have been studied. For the first time, the toxicological properties of fullerenes C60 have been studied when they were co-administered with the toxicant toluene. It has been established that carbon nanoparticles fullerenes C60 enhance the hepatotoxic effect of toluene (activities of trasaminases and phosphatases are increased), increase the intensity of oxidative and nitrooxidative stress caused by toxicant (the processes of lipoperoxidation and oxidative modification of proteins are activated, the antioxidant system is supressed, the activity of NO-synthase and NOx content are increased), increase the production of intracellular ROS, the content of apoptically and necrotically altered neutrophils in the blood caused by toluene, enhance the negative effects of toluene on the immune system (the concentration of TNF-α, IL-1β, IL-6, immunoglobulins A, M and G are increased, the content of IL-4 and IL-10 are decreased) and the functioning of enzymes of xenobiotics biotransformation. Fullerenes C60 also enhance the ability of toluene to cause structural changes in the tissues of the liver, kidneys and spleen.