Blokhina O. Biochemical parameters of blood under the development of experimental joint inflammation and chondroitin sulfate prophylactical addition.

The thesis for candidate of biological science degree is devoted to the research of chondroitin sulfate prophylactic action onto the rat blood biochemical parameters in the case of carrageenan-induced joint inflammation. During the carrageenan-induced joint inflammation in whole organism the development of inflammation and endogenic intoxication were observed: the increase in levels of pro-inflammatory cytokines (IL-1β, IL-12В р40, TNF-α), C-reactive protein concentration and low molecular mass molecules amount are evident, while the levels of anti-inflammatory cytokines (IL-4, IL-10) remain unchanged. It was also shown that in the blood under these experimental conditions there was the expression level increase of the genes, taking part in inflammation development (Ptgs2, Nos2, Tgfb1, Nfkb1). In the case of prolonged prophylactic injection of chondroitin sulfate to rats with joint inflammation the beforementioned parameters become normal, while the level of IL-10 was increased in comparison with the animal group with the pathology. During experimental joint inflammation intensification of free radical formation process in the rat blood was detected: the amounts of superoxide, hydrogen peroxide and lipid and protein oxidation products were increased. Under these experimental conditions the blood antioxidant system function was compromised: the activity of superoxide dismutase, glutathione peroxidase and glutathione reductase as well as the amount of reduced glutathione were decreased, while the catalase and glutathione transferase activity, as well as the amount of oxidized glutathione, were found to be increased as compared to the controls. During the prophylactic injection of chondroitin sulfate to rats with experimental joint inflammation oxidative homeostasis restoration was observed as opposed to the animal group with carrageenan-induced joint inflammation. The data obtained indicates immuno-modulative, anti-inflammatory and antioxidative properties of investigated chondroitin sulfate based preparation on the whole organism during experimental joint inflammation.