Pastoienko D. Population-genetic adaptability of intarbreed types of Ukraine carp.

Українська версія

Thesis for the degree of Candidate of Sciences (CSc)

State registration number

0421U103287

Applicant for

Specialization

  • 03.00.16 - Екологія

27-04-2021

Specialized Academic Board

Д 26.371.01

Institute of Agroecology and Environmental Management of National Academy of Agricultural Sciences of Ukraine

Essay

The dissertation is devoted to the study of adaptation and effective development of carp intrabreed types populations in aquaculture of Ukraine basing on scientific substantiation ecological conditions and genetic features of ontogenesis optimization. The ecological peculiarities of intra-breed types of carp of different genesis and their adaptability in ecological-geographical zones of fish farming of Western Ukraine, in which they reproduce, were clarified in the work. The ecological condition of reservoirs is assessed, the species composition of biota, natural forage base and factors of influence of the natural environment of aquaculture conditions are determined. The phenotypic variability of the Antoninsko-Zozulynetskyi intrabreed type of scaly and farmed carp breeds was determined. Peculiarities of isoenzyme and protein spectra have been established and a comparative analysis of the genetic diversity of the nuclear genome of the Antoninsko-Zozulynetskyi intrabreed type of carp using the ISSR-PCR method has been performed. The frequency o allelic variants, the average level of heterozygosity, genetic distances in carp according to the studied molecular genetic markers were analyzed. The polymorphism of genes of different carp that control the expression of genetic-biochemical and DNA markers was studied, their informativeness and possibilities of using the obtained data to control structural changes of biodiversity and ensuring homeostasis balance in fish farming ecosystems were evaluated. Criteria for selection process assessment and genetic monitoring of carp populations adaptability to environmental conditions and industrial cultivation technologies are substantiated.

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